@article{copb2015rnastructrev,
	Abstract = {\{RNAs\} fold into intricate and precise secondary structures. These structural patterns regulate multiple steps of the \{RNA\} lifecycle, while also conferring catalytic and scaffolding functions to certain transcripts. Therefore, a full understanding of \{RNA\} posttranscriptional regulation requires a comprehensive picture of secondary structure. Here, we review several high throughput sequencing-based methods to globally survey plant \{RNA\} secondary structure. These methods are more accurate than computational prediction, and more scalable than physical techniques such as crystallography. We note hurdles to reliably measuring secondary structure, including RNA-binding proteins, \{RNA\} base modifications, and intramolecular duplexes. Finally, we survey the functional knowledge that has been gleaned from each of these methods, and identify some unanswered questions that remain. },
	Author = {Shawn W Foley and Lee E Vandivier and Pavel P Kuksa and Brian D Gregory},
	Bib2Html_Pubtype = {Journal},
	Date-Added = {2015-12-28 18:44:36 +0000},
	Date-Modified = {2020-03-06 13:18:28 -0500},
	Doi = {http://dx.doi.org/10.1016/j.pbi.2015.05.021},
	Issn = {1369-5266},
	Journal = {Current Opinion in Plant Biology},
	Note = {Cell signalling and gene regulation},
	Pages = {36 - 43},
	Title = {Transcriptome-wide measurement of plant {RNA} secondary structure},
	Url = {http://www.sciencedirect.com/science/article/pii/S1369526615000722},
	Volume = {27},
	Year = {2015},
	Bdsk-Url-1 = {http://www.sciencedirect.com/science/article/pii/S1369526615000722},
	Bdsk-Url-2 = {http://dx.doi.org/10.1016/j.pbi.2015.05.021}}